Characterization of Pyruvate Uptake in Escherichia coli K-12
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چکیده
منابع مشابه
Characterization of Pyruvate Uptake in Escherichia coli K-12
The monocarboxylate pyruvate is an important metabolite and can serve as sole carbon source for Escherichia coli. Although specific pyruvate transporters have been identified in two bacterial species, pyruvate transport is not well understood in E. coli. In the present study, pyruvate transport was investigated under different growth conditions. The transport of pyruvate shows specific activiti...
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Pyruvate is an important intermediate of central carbon metabolism and connects a variety of metabolic pathways in Escherichia coli Although the intracellular pyruvate concentration is dynamically altered and tightly balanced during cell growth, the pyruvate transport system remains unclear. Here, we identified a pyruvate transporter in E. coli using high-throughput transposon sequencing. The t...
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A mutant of Escherichia coli K-12 lacking pyruvate dehydrogenase and phosphoenolpyruvate synthase was used to study the transport of pyruvate by whole cells. Uptake of pyruvate was maximal in mid-log phase cells, with a Michaelis constant for transport of 20 microM. Pretreatment of the cells with respiratory chain poisons or uncouplers, except for arsenate, inhibited transport up to 95%. Lactat...
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SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0067125